Plasma level of TNF-α in PBC patients (3.90(3.90 ~ 42.65) pg/ml) after TN combined with UDCA (less than 4 pg/ml) showed a trend of decrease (P = 0.068), which was not seen after sham-TN combined with UDCA (3.90(3.90 ~ 14.70)pg/ml) (P > 0.05). The gene discussed is TNF; the disease is primary biliary cholangitis.