Finally by employing a preclinical mouse model of NB, obtained by implantation of IMR-32 cells into mice adrenal glands, we demonstrated that after Roniciclib administration, NPM1 and PES1 translocate from nucleolus to the nucleoplasm and cytoplasm in vivo too and, in particular, we confirmed an impressive reduction in tumor growth, indicating promising preconditions for the choice of this drug in NB treatment. Here, NPM1 is linked to neuroblastoma.