This study proposes the involvement of several key nucleolar proteins, such as NCL25–28, Nucleophosmin-1 (NPM1)40, Glypican-2 (GPC2)41, and Pescadillo Ribosomal Biogenesis Factor-1 (PES1)42 in the nucleolar stress induced by Roniciclib in both stem and non-stem NB cells. This evidence concerns the gene NPM1 and neuroblastoma.