Immunoblotting analysis revealed that enhanced phosphorylation of p65 (according to the phosphorylated/total ratio, P/T ratio) was detected in the normoxic CRC cells treated with the medium enriched in IL-8, such as HSS-CM and rhIL-8, and a decreased P/T ratio was detected when cells were treated with medium depleted of IL-8, such as siIL-8-HSS-CM and HSS-CM + anti-IL-8 (Fig. 4a). This evidence concerns the gene CXCL8 and colorectal carcinoma.