MAPT and Alzheimer disease: After 24-h treatment with 0.25 μm oligomeric tau from DLB, AD, and PSP, alone or in the presence of CL3 (final concentration 5 μm), total cell lysates were collected using 1× radioimmune precipitation assay buffer (Cell Signaling, 9806) supplemented with 2% protease/phosphatase inhibitor (Roche Applied Science, 04906837001).