Further, CFPS TNF-α, IFN-γ, and IL10 responses singly and in combination had high NPVs for both co-prevalent TB (97–99%) and incident TB (99–100%), indicating a potential for using these responses as a tool to identify a subgroup of contacts with a high certainty of not currently having TB nor of being diagnosed with TB in the future. The gene discussed is TNF; the disease is tuberculosis.