In contrast, E-cad and ZO-1 expression at the sites of cell-cell contacts are highest in the different types of cancer cells cultured on TMG, a bit lower in the Col I culture groups, and lowest in the Matrigel cultures (Fig. 4, middle and right panels) although the cancer cells tended to be clustered together in the Col I and Matrigel cultures. The gene discussed is CDH1; the disease is cancer.