We transduced Cas9 expressing Gsdmd–/–;Bid–/–;Mlkl–/–;Casp3–/–;Casp7–/–;Casp9–/– iBMDMs with a whole-genome single guide RNA (sgRNA) library (Koike-Yusa et al., 2014) and stringently enriched for sgRNAs that promoted cell survival after Salmonella infection by repeating the infection and selection procedure three times. Here, MLKL is linked to infection.