GSDMD and infection: We transduced Cas9 expressing Gsdmd–/–;Bid–/–;Mlkl–/–;Casp3–/–;Casp7–/–;Casp9–/– iBMDMs with a whole-genome single guide RNA (sgRNA) library (Koike-Yusa et al., 2014) and stringently enriched for sgRNAs that promoted cell survival after Salmonella infection by repeating the infection and selection procedure three times.