At an MOI of 1, both vCyclin and lytic antigen expression was detected at 12 hr and 24 hr post-infection and the expression levels were comparable between WT and IL16 KO MEFs; however, at an MOI of 0.05, MHV68 lytic gene expression detected by lytic serum was slightly reduced in IL16 KO MEFs compared to WT MEFs at 36 hr and 48 hr post-infection (Fig 3A). This evidence concerns the gene IL16 and infection.