RPSA and familial pancreatic carcinoma: For this reason, MIA PaCa-2 pancreatic cancer cells were pre-incubated with EGCG (10 μM) for 1 h, then incubated with or without AG-9 (10–7 M) for 24 h before fixation with paraformaldehyde and labeling with anti-TRPM7 and RPSA antibodies.