Here, to verify the functional deficiency of CST3 in Cst3-KO gerbils and to assess the role of CST3 in stroke, we ligated unilateral carotid arteries of gerbils, and found that the brain infarct areas of Cst3-KO gerbils (23.2 ± 1.91%) were significantly larger than those of WT gerbils (15.6 ± 1.75%) by the TTC staining assay (Figures 4A,B). This evidence concerns the gene CST3 and Stroke.