With the transfection of LKB1 siRNA, LPCAT2 siRNA, or AMPK activator, we proved that LKB1 was the upstream regulator of AMPK and AMPK was the upstream factor of ELAVL1 and LPCAT2, with the treatment of LKB1 siRNA, AMPK siRNA, and angustoline, we further validated that angustoine inhibited esophageal tumor through activating LKB1 and AMPK, then suppressing ELAVL1, and LPCAT2. This evidence concerns the gene PRKAA2 and neoplasm of esophagus.