Considering the study on the effects of angustoline on esophageal tumor was rarely reported, the present manuscript investigated anti-tumor role of angustoline in vitro and in vivo, and explored the regulation of angustoline in LKB1/AMPK/ELAVL1/LPCAT2 pathway and phospholipid remodeling. This evidence concerns the gene PRKAA1 and neoplasm of esophagus.