Our data show that TMEM165KO in HEK293T and HeLa cells display NCSTN glycoforms with migration to a lower molecular weight than the MDAMB231 breast cancer TMEM165KO cells (Figure 5B) These results indicate that the NCSTN glycoprotein from MDAMB231 breast cancer cells have different glycosylation compared with HEK293T and HeLa cells. This evidence concerns the gene NCSTN and breast cancer.