In this study, we selected eight lncRNAs (HULC, MALAT1, Linc00152, PTENP1, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1) from previously published HCC-related studies [11, 14, 15, 18–20] and assessed their expressions in serum of 129 HCC patients, 49 patients with LC, 27 patients with CHB, and 93 healthy controls. Here, SPRY4 is linked to laryngotracheoesophageal cleft.