In contrast to the in vitro results with the DHFR activity model, the inhibition of granuloma formation in the cotton pellet test, a model for chronic proliferative inflammation, was stronger with STW1 than with the single herbal extracts, but not significant versus control, while the antiproliferative effect in adjuvant-induced arthritis was significantly superior to control. The gene discussed is DHFR; the disease is Arthritis.