Thus, we determined whether local C5aR1 activation affects the transcription of IFN-γ (Ifng) in the spleen and brain as well as IL-12p35 (Il12a), IL-12p40 (Il12b), IL-18 (Il18), and iNOS (Nos2) in the brain of T. gondii-infected animals on day seven after infection using RT-qPCR (Figures 5A–F). This evidence concerns the gene NOS2 and infection.