Breast cancer cells incubated with pre-coated HA, HA + rfhSP-D, and rfhSP-D-only wells, along with untreated (cells only) cells, were stained after 24 h with Annexin V/FITC; the quantification of apoptosis was carried out using flow cytometry (Figure 6A). This evidence concerns the gene ANXA5 and breast cancer.