Our findings suggest that higher levels of DNAJC27 can reduce wild-type MC4R signaling in vitro, however, we still cannot make a direct link between the circulating levels of DNAJC27 and MC4R signaling in the hypothalamus and further studies are now required to understand how DNAJC27 could affect the appetite and the eventual development of obesity. This evidence concerns the gene MC4R and obesity due to melanocortin 4 receptor deficiency.