The other 80 studies conducted in vitro experiments (Figure 2E), 57 (71.3%) of them used only mushroom tyrosinase, 13 (16.3%) of them used only mice cell lines (mostly B16 cells) and human melanoma cell lines, with or without mushroom tyrosinase; only 9 studies used human epidermal melanocytes (HEMs) and 3-dimensional human skin equivalents (3D skin). Here, TYR is linked to melanoma.