To further explore the role of EN2 in CRC and the mechanisms of its oncogenic function, we screened the EN2 co-expressed genes from GSE9348 (|R| ≥ 0.3) and then performed KEGG pathway analysis on those genes, among which some positively correlated with EN2 enriched in cell cycle, RNA transport and oocyte meiosis, while others negatively correlated with it enriched in metabolic pathways, biosynthesis of antibiotics and fatty acid degradation (Fig. 2a, b). This evidence concerns the gene EN2 and colorectal carcinoma.