INS and Hyperglycemia: Fasting blood glucose more than 15 mM for 30 days and depleted serum insulin levels confirm the model establishment for ß-cell death (Fig. 2c, d) Histo-morphological assessment of pancreatic sections stained with hematoxylin and eosin (H + E) and immunohistochemistry for insulin (red) showed pancreatic injury and ß-cell damage in islets at day 30, resulting in hypoinsulinemia and hyperglycemia (Fig. 2f, g).