Having shown that PRMT5 is further overexpressed at the transcriptional level in the KRAS mutant CRC cells when compared to the KRAS WT CRC cells, a Western blot assay was then performed to ascertain whether this overexpression of PRMT5 at the transcriptional level affected a further overexpression of PRMT5 at the translational level in the KRAS mutant CRC cells. The gene discussed is KRAS; the disease is colorectal carcinoma.