For this reason, we co-transfected the SH-SY5Y cells with either TDPwt, dNLS, dNLSd343 or KO2only with selected wild-type (wt) or mutant (mut) ALS-linked gene tagged with 3xHA: hnRNPA1, MATR3, VCP or UBQLN2. These genes were selected based on their diverse roles in RNA processing and quality control of protein metabolism. This evidence concerns the gene UBQLN2 and amyotrophic lateral sclerosis.