As shown in Fig 4, using a cutoff of 29.88 ng/ml for OPN and 47.63 pg/ml for CXCL10, they do distinguish clearly between the presence or absence of NAFLD when adjusted for both age and gender and may represent a useful diagnostic tool to augment AST/ALT tests in subjects with mild NAFLD enabling early diagnosis and intervention. Here, SPP1 is linked to metabolic dysfunction-associated steatotic liver disease.