POLE and breast carcinoma: We calculated the mutation rates of the top 40 mutated genes (Figure 1A), imprinted genes (PEG3, UBE3A, GRB10, PEG10, SNRPN, NDN) and genes that might affect the mutation rates of the genome (BRCA1, BRCA2, POLE, MLH3) (Figure 1B) respectively, and the result revealed that PEG3 and BRCA2 presented relatively high mutation rates in the breast cancer samples.