Here, we examined the status of EGFR, HER2, BRAF, KRAS, ALK, MET, NRAS, PIK3CA, RET, and ROS1 in 884 patients with NSCLC who had undergone surgical resection, bronchoscopy biopsy or percutaneous transthoracic biopsy by NGS in our institution. This evidence concerns the gene ALK and non-small cell lung carcinoma.