Similar to our in vitro results, immunohistochemical analyses of the lungs of KRASLA2 mice showed decreased pAKT and increased TRIB3 expression in tumor mass of PIERCE1 KO mice (Supplementary Fig. 13b), implying that ablation of PIERCE1 suppresses lung tumorigenesis with impaired AKT activity in vivo. This evidence concerns the gene TRIB3 and neoplasm.