In this study, we observed that 1) serum VEGF-A and VEGF-D levels were suppressed at 1 week after IVC and returned to baseline at 4 weeks, while no significant differences were observed in the serum levels of other cytokines between baseline and 1 week or 4 weeks after IVC injection; 2) compared with the control group, ROP patients had significantly different serum concentrations of several angiogenic, inflammatory, and chemotactic factors, indicating that those factors may be associated with ROP. Here, VEGFD is linked to retinopathy of prematurity.