Another important difference in the proportion of mixed infections detected by RDT type 3 compared with microscopy was also demonstrated in the study conducted in Madagascar by Mehlotra et al., 2019 during 2015–2016 because 84.6% of blood samples with confirmed P. falciparum mono-infections by microscopy and by LDR-FMA analysis were positive for both the Pf-HRP2 and pan-pLDH test bands44. The gene discussed is HDGFL2; the disease is infection.