We found that CM from PBMC stimulated with a dual-acting agonist of TLR7/8 (R848) and TLR2/7 (CL413) were more potent drivers of inhibition of hepatitis e and s antigens (HBeAg and HBsAg) production from HBV-infected PHH than CM from PBMCs stimulated with agonists specific only for TLR7 (GS-9620, CL264) or TLR9 (CpG-A, CpG-B). Here, TLR7 is linked to hepatitis A virus infection.