Trp53, a known tumor suppressor in human LGG and GBM [33], was the second most frequently mutated gene (5/17 tumors had a Trp53 missense mutation, all within Trp53’s DNA-binding domain; p = 1.13 × 10−12, FDR 7.75 × 10−9, LRT; Additional file 1: Fig. S9), validating the application of WES to identify relevant collaborative mutations. The gene discussed is TP53; the disease is glioblastoma.