RB1 and brain glioma: Gene set enrichment analysis (GSEA) of differentially expressed genes in EGFRvIII-brain gliomas showed significantly enriched gene sets (p < 0.01) including p53, Wnt, MAPK, Jak-Stat, Rb pathways, and stemness, implicating these oncogenic pathways in driving gliomagenesis in cooperation with EGFRvIII (Fig. 2c, Additional file 1: Fig. S11).