Moreover, 16 of the top 20 CIS genes had supporting fusion transcripts from at least one tumor, including Cdkn2a, Nf1, Pten, Sox6, Sox5, Spred1, and Tcf12 (all containing PB splice acceptor fusions, implying transcript termination; Fig. 6d). This evidence concerns the gene SPRED1 and in situ carcinoma.