Mice later developed neurological signs due to one or multiple gliomas within the lateral ventricles and/or brain surface with evidence of subarachnoid involvement (40/48 mice had brain gliomas; mean survival 41.1 weeks); immunostaining for human EGFR and EGFRvIII confirmed EGFRvIII expression specifically and clonally in tumor cells of microneoplasias and gliomas (Additional file 1: Fig. S3, S4). Here, EGFR is linked to brain glioma.