To explore whether the Wnt/β-catenin pathway and autophagy were involved in the gemcitabine resistance induced by PVT1 in human pancreatic cancer cells, we investigated the protein and mRNA expression levels of the Wnt/β-catenin target genes CyclinD1, C-myc and Axin2 after PVT1 overexpression or knockdown. The gene discussed is CCND1; the disease is familial pancreatic carcinoma.