The aims of this study were, first, to investigate how the expression of formins is altered in migrating glioblastoma cells in an in vitro model, second, to evaluate the importance of selected formins for migration in functional studies, and third, to study the expression of FHOD1 (formin homology domain containing protein 1) and INF2 (inverted formin 2) in human glioblastoma specimens. Here, FHOD1 is linked to glioblastoma.