In this study, we identified that EZH2 and DLC1 were negatively correlated in BC based on TCGA database analysis (R = −0.13, P = 2.7 × 10−5) and further confirmed that EZH2 and DLC1 were negatively correlated in BC patients by collecting clinical samples (R = −0.248, P = 0.013). The gene discussed is DLC1; the disease is breast cancer.