The basal levels of Pip in B. distachyon leaves, at 1–2 μg g-1 FW, were higher than those generally observed for Arabidopsis Col-0 leaves (Návarová et al., 2012), and Pip levels started to accumulate significantly only at later stages of infection (5 dpi), to ~4 μg g–1 FW (Fig. 5A). This evidence concerns the gene PIP and infection.