Melatonin reversed HFD-caused liver fibrosis and NAFLD by increasing the ratio of LC3-II/I, decreasing cytoplasmic p62 levels and reducing the expression of miR-34a-5p in WT, but not SIRT1 heterozygous (HET) mice 64. This evidence concerns the gene SQSTM1 and metabolic dysfunction-associated steatotic liver disease.