WNT3A and neoplasm: Culturing dissociated single tumour cells in Matrigel with a cocktail medium of niche factors (WNT-3A, R-Spondin, etc.)enabled us to develop ovarian cancer organoids from different histologic subtypes (HGSC, EM, CCC) of stage I–III ovarian cancer patients within 1–3 weeks (Fig. 1A, Table 1).