The preclinical studies showed that nutlin-3 increases p53 concentration and induces apoptosis or senescence in numerous, mostly WT p53 cell lines of osteosarcoma38,42, lung cancers43, prostate cancers44, colon cancer42, melanoma45, ovarian cancer46, neuroblastoma47 and renal cancer48. This evidence concerns the gene TP53 and digestive system neoplasm.