To further delineate the role of BDNF in the NLC microenvironment, we artificially generated an NLC secretome by combining human cytokines BAFF (+hBAFF), APRIL (+hAPRIL), and CXCL12 (+hCXCL12) and applied them to isolated B-CLL cells. Here, CXCL12 is linked to B-cell chronic lymphocytic leukemia.