Four of the PBC-associated clusters were not members of PBC-associated metaclusters; these include: (1) Cluster-50 (Metacluster-9), a subset of CD14+CD16+ monocytes expressing high levels of IL3R and TIM3; (2) Cluster-68 (Metacluster-9), a subset of monocytes with low CD14 expression; (3) Cluster-113 (Metacluster-9), a subset of CD66b+ cells (contaminating granulocytes); and (4) Cluster-196 (Metacluster-18), a subset of CD45− cells expressing B cell markers (CD19 and CD20). This evidence concerns the gene CEACAM8 and primary biliary cholangitis.