Unsupervised clustering analysis was adopted and found that the expression levels of some immune cells between clusters, such as “Mast cell resting (P < 0.001)”, “T cell regulatory (P < 0.01)”, “Macrophages M1 (P < 0.01)”, “Eosinophils (P < 0.01)”, and “T cell CD4 memory resting (P < 0.01)”, were significantly different, demonstrating that differences in CAAS could lead to changes in the tumour immune microenvironment. The gene discussed is CD4; the disease is neoplasm.