Moreover, cyclopamine, an canonical Hh signaling inhibitor targeting SMO, repressed the expression of TPX2 in protein level and mRNA level in multiple HCC cells, as well (Fig. 1d-e, and S1h-i), which further indicates that pharmacological attenuation of Hh signaling decreases the expression of TPX2. This evidence concerns the gene TPX2 and hepatocellular carcinoma.