To further confirm the regulation of TPX2 in HCC cells, the protein level of TPX2, together with its mRNA level, were determined in Hep3B, another HCC cell line, and results revealed that GANT61 would decrease both the mRNA level (Fig. 1c) and protein level (Fig. 1d) of TPX2 in Hep3B cells. Here, TPX2 is linked to hepatocellular carcinoma.