To elucidate the biological effect of TPX2 on Hh/GLI signaling-dependent growth of HCC cells, HepG2 and SK-Hep-1 cells, in which the expression level of TPX2 was relatively lower (Fig. S7d), were infected by lentivirus to generate cell lines with TPX2 stably expressing (Fig. 2a-b and S2a). The gene discussed is GLI1; the disease is hepatocellular carcinoma.