For example, DGKζ binds directly to Rac1 to form a complex with Rho-GDI and PAK1 [93], DGKβ co-localized with actin filaments [94], whereas endogenous DGKζ co-purified with cytoskeletal proteins and localized to the leading edge of C2 myoblasts [42] and glioblastoma cells [58]. This evidence concerns the gene DGKB and glioblastoma.