In our quest to find a suitable phenotypic assay for potential OGT inhibitors and to study in-depth cancer and immune cellular response following OGT inhibition, we have studied the effect of one of these OGT inhibitors (OSMI-4 in its free acid and ester forms) in selected cell lines (K562, HEK293 and HEK293T). The gene discussed is OGT; the disease is cancer.