A lower outcome is obtained for 68Ga-DOTA-NMB and 68Ga-DOTA-NMB-AuNP on colon cancer cell line HT-29, for which the uptake profile follows an up and down trend, an indicator of unstable binding to neuromedin B receptors (NMB, BBRS2, BBRS3). Here, NMBR is linked to colonic neoplasm.