Overall, our data from CRC, cervical and prostate cancer cell lines lead us to conclude that glutamine depletion or inhibition of Akt/mTORC1 signalling induces a switch in the balance of exosome secretion towards vesicles from recycling endosomal compartments, increasing exosome levels of Rab11a, as well as Cav‐1, by several fold. This evidence concerns the gene RAB11A and prostate cancer.