Metabolite profiles were found to be highly specific with only a few metabolites significantly associated with more than one variant, such as 3-methylglutarylcarntine (PNPLA3 and HSD17B13) and lysoPC C14:0 (TM6SF2, HSD17B13 and MTARC1) that were not replicated in other variants associated with liver fat but not fibrosis (e.g. LYPLAL1) or fibrosis without steatosis (e.g. SERPINA1). This evidence concerns the gene LYPLAL1 and steatosis.