Metabolite profiles were found to be highly specific with only a few metabolites significantly associated with more than one variant, such as 3-methylglutarylcarntine (PNPLA3 and HSD17B13) and lysoPC C14:0 (TM6SF2, HSD17B13 and MTARC1) that were not replicated in other variants associated with liver fat but not fibrosis (e.g. LYPLAL1) or fibrosis without steatosis (e.g. SERPINA1). The gene discussed is SERPINA1; the disease is steatosis.