TP53 and neoplasm: One mechanism for a high VAF maybe loss of heterozygosity (LOH) in the tumour.41 In keeping with this, a number of tumours have borderline evidence for shallow loss of TP53 in the nanostring nCounter data (CRB59: TP53 Tumour VAF 62.2%, CN 1.5 and CRB67: TP53 Tumour VAF 54.2, CN 1.5 (Supplementary Fig. 9)).