BRCA2 and melanoma: We have confirmed the regulation of four exemplary genes, namely RAD51, BRCA1, BRCA2, and XRCC2 by vemurafenib treatment in further BRAF-mutated melanoma cell lines using RT-qPCR analysis at the mRNA level (Fig. 2b) and the regulation of Rad51 at protein level using immunoblot analysis (Fig. 2c).