For this, we made use of a protocol that was previously established in our lab to isolate tongue T cells, which are rare in this intractable organ.41,52 Starting from 1 week post infection, a small but distinct population of CD4+ T cells was detected in the tongue of mice infected with C. albicans strain 101 or strain SC5314 (Fig. 2a, b). The gene discussed is CD4; the disease is infection.