BRCA1 and neoplasm: When we combined both clinically screened and unscreened SCAN-B patients (n = 105 in total, reanalyzed using the same instrument settings and reagent lots), we observed that the higher BRCA1 blood DNA methylation levels appeared independent of age (two-way ANOVA interaction model with tumor hypermethylation status and age groups, p = 0.002, Fig. 3c).